ABSTRACT
Salvia miltiorrhiza is one of the most common traditional Chinese medicines. It has rich resources in China. According to modern studies, phenolic acids are the main effective components in S. miltiorrhiza. These components have cardiovascular and cerebrovascular protective effect, and anti-tumor, antioxidant, anti-inflammatory, and antifibrotic activities, etc. It has been widely used for the treatment of cardiovascular and cerebrovascular diseases and others. In this paper, the chemicals and pharmacological effects of phenolic acids from S. miltiorrhiza were summarized in the last decade. Its researches and development prospects were also analyzed for further studying and comprehensive utilization of these phenolic acids.
ABSTRACT
This study is to establish the fingerprint for Phyllanthus emblica and their tannin parts from different habitats by HPLC for its quality control. The determination was carried out on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column, with methanol-0.2% glacial acetic acid as mobile phase with gradient elution at a flow rate of 1 mL x min(-1). The temperature was maintained at 30 degrees C and the detected wavelength is 260 nm, Thirteen chromatographic peaks were extracted as the common peaks of the fingerprint of P. emblica, and eleven as the common peaks of P. emblica tannin parts, and five peaks were identified by comparing with referent samples. The fingerprints of 8 samples were compared and classified by similarity evaluation, cluster analysis and principal component analysis (PCA). The similarity degrees of eight P. emblica were between 0.763 and 0.993, while tannin parts were between 0.903 and 0.991. All the samples of P. emblica and their tannin parts were classified into 3 categories. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of P. emblica from different habitats.
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Medicine, Tibetan Traditional , Phyllanthus emblica , Chemistry , Classification , Quality Control , Tannins , TibetABSTRACT
To study the constituents of the Prunella vulgaris L, the constituents were isolated by various column chromatography and the structures were identified on the basis of chemical and spectral analysis. One saponin compound (I) and one flavone glycoside compound (II) were obtained from Prunella vulgaris L. Their structures were elucidated as 16-oxo-17-demethyl-3beta,24-dihydroxylolean-12-en-3-O-beta-D-glucuronoside (I), and acacetin-7-O-beta-D-glucopyranoside (II). Compound I is a novel triterpenoid saponin and named as prunelloside A. Compound II was obtained for the first time from the Prunella genus.
Subject(s)
Flavonoids , Glucosides , Molecular Structure , Plants, Medicinal , Chemistry , Prunella , Chemistry , Saponins , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To establish methods for the determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC.</p><p><b>METHOD</b>The determination of phillyrin was carried out on YWG-C18 column (4.6 mm x 250 mm, 10 microm), using acetonitrile-water (25:75) as mobile phase at a flow rate of 0.8 mL x min(-1) and detected at the wavelength 277 nm. The determination of forsythoside A was carried out with YWG-C18 column(4.6 mm x 250 mm,10 microm), using acetonitrile-water-Acetic acid (17:83:0.4) as mobile phase at a flow rate of 1.0 mL x min(-1) and detected at the wavelength 280 nm.</p><p><b>RESULT</b>The average recovery of phillyrin was 99.6%, RSD = 1.9% (n = 5). The average recovery of forsythoside A was 101.3%, RSD = 2.5% ( n = 5).</p><p><b>CONCLUSION</b>The methods were simple and accurate and could be used to control the quality of the Lianqiao Bingdu Qing capsule.</p>
Subject(s)
Capsules , Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Forsythia , Chemistry , Fruit , Chemistry , Glucosides , Glycosides , Plants, Medicinal , ChemistryABSTRACT
<p><b>AIM</b>To investigate the chemical constituents of Phyllanthus urinaria L.</p><p><b>METHODS</b>Various chromatographic techniques were employed for the isolation and purification. The structure was elucidated by spectral analyses.</p><p><b>RESULTS</b>A novel ellagitannin named phyllanthusiin G was isolated, its structure was established as 1-O-galloyl-2-phyllanthoyl-3,6-(R)-HHDP-beta-D-glucose.</p><p><b>CONCLUSION</b>Phyllanthusiin G is a new compound.</p>
Subject(s)
Molecular Conformation , Molecular Structure , Phyllanthus , Chemistry , Plants, Medicinal , Chemistry , Tannins , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of Tibetan medicine Phyllanthus emblica.</p><p><b>METHOD</b>Various chromatographic techniques were employed for isolation and purification of the constituents, and the structures were elucidated by chemical and spectral analyses.</p><p><b>RESULT</b>11 compounds were isolated and identified as gallic acid (I), ellagic acid (II), 1-O-galloyl-beta-D-glucose (III), 3,6-di-O-galloyl-D-glucose (IV), chebulinic acid (V), quercetin (VI), chebulagic acid (VII), corilagin (VIII), 3-ethylgallic acid (3-ethoxy-4,5-dihydroxy-benzoic acid, IX), isostrictiniin (X), 1,6-di-O-galloyl-beta-D-glucose (XI).</p><p><b>CONCLUSION</b>3-Ethylgallic acid (3-ethoxy-4,5-dihydroxy-benzoic acid) is a novel compound, and isostrictiniin was found from P. emblica for the first time.</p>